Holy of Holies, the innermost and most sacred area of the ancient Temple of Jerusalem, accessible only to the Israelite high priest. Once a year, on Yom Kippur, the Day of Atonement, he was permitted to enter the square, windowless enclosure to burn incense and sprinkle sacrificial animal blood. By this act, the most solemn of the religious year, the high priest atoned for his own sins and those of the priesthood.
The Holy of Holies was located at the west end of the Temple, and in Solomon’s Temple it enshrined the Ark of the Covenant, a symbol of Israel’s special relationship with God. At the entrance to the Holy of Holies stood a small cedar altar overlaid with gold. After his conquest of Jerusalem in 63 bc, Pompey desecrated the Temple by daring to enter the Holy of Holies. See also Jerusalem, Temple of.