Charles de Biencourt (born 1591/92, Champagne, Fr.—died 1623/24, Port-Royal, Acadia, New France [now in Canada]) was a French colonizer who commanded the French colony of Port-Royal.
In 1606 Biencourt sailed with his father, Jean de Biencourt de Poutrincourt, baron de Saint-Just, to Acadia. In 1607 they abandoned their establishment and fort at Port-Royal, Acadia, because of insufficient funds. Failing to obtain a Canadian fur-trade monopoly while in France, father and son returned to Acadia and to Port-Royal in 1610. In 1611 Jean de Biencourt was appointed vice admiral of the seas of New France; he placed Port-Royal under his son’s administration.
Charles was not effective in exerting authority, especially over the Jesuits. In 1613–14 the British, under Sir Samuel Argall, attacked Port-Royal. The elder Biencourt returned to the devastated settlement and made over his holdings to his son, who stayed on with a few colonists. Charles and his colleagues built up the fishing and fur-trading business there and established a new company. In 1618 Charles appealed unsuccessfully to Paris for fortification against the English. The settlement did not prosper, and Biencourt lived with Native Americans (First Nations) during his last years.