sequence determination

…developed technologies capable of deep-coverage sequencing, in which DNA segments were read rapidly multiple times to ensure that the determined order of bases was accurate. The two projects based on deep coverage, which enhanced the ability to detect low-frequency mutations, involved genome sequencing of a small number of trios (a…

Genomic sequences are usually determined using automatic sequencing machines. In a typical experiment to determine a genomic sequence, genomic DNA first is extracted from a sample of cells of an organism and then is broken into many random fragments.…

Methods to determine the sequences of bases in DNA were pioneered in the 1970s by Frederick Sanger and Walter Gilbert, whose efforts won them a Nobel Prize in 1980. The Gilbert-Maxam method relies on the different chemical reactivities of the bases, while the…