Among the mammals possessing echolocation are the toothed whales. These animals probably produce sounds in the water in two ways: with the larynx and with the complex system of passages connected to the blowhole, which is a nostril in the top of the head. Although many different types of sounds are possible, during echolocation they consist mainly of a rapid series of clicks. These clicks contain many components, but the principal energy is in the high frequencies, from perhaps 50,000 to as much as 200,000 hertz. The use of such high frequencies by these animals is a requirement for effective echolocation in water. Because the velocity of sound is greater in water than in air, the wavelengths are longer; therefore, in order for echolocation to attain the same effectiveness of object discrimination as that achieved by the bat with aerial sounds, an aquatic animal has to use frequencies at least five times as high.

Whales have good vision when submerged, and apparently their eyes remain fairly serviceable when their heads are out of water. Dolphins can be trained to strike targets or leap over obstacles held several feet above the surface of the water. For many tasks, however, they use echolocation very effectively, such as when catching fish at night or when visibility is poor in murky water. Dolphins have been trained to make fine discriminations of objects when their vision has been completely excluded by blindfolding. Echolocation of some form and degree of effectiveness is suspected in still other animals, such as shrews and sea lions, but the evidence is meagre thus far.